{"id":13870,"date":"2021-04-07T13:03:44","date_gmt":"2021-04-07T18:03:44","guid":{"rendered":"https:\/\/wildlifepreservation.qa.caorda.com\/blog\/?p=13870"},"modified":"2023-04-18T12:02:37","modified_gmt":"2023-04-18T17:02:37","slug":"breeding-season-science","status":"publish","type":"post","link":"https:\/\/wildlifepreservation.ca\/fr\/blog\/breeding-season-science\/","title":{"rendered":"Breeding season science"},"content":{"rendered":"<p><div id='av_section_1'  class='avia-section av-1j97qu8-d954dbdec10ff5de904045a47b708e65 main_color avia-section-default avia-no-border-styling  avia-builder-el-0  el_before_av_one_full  avia-builder-el-first  avia-bg-style-scroll container_wrap fullsize'  ><div class='container av-section-cont-open' ><main  role=\"main\" itemprop=\"mainContentOfPage\"  class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-13870'><div class='entry-content-wrapper clearfix'><\/div><\/div><\/main><!-- close content main element --><\/div><\/div><div id='after_section_1'  class='main_color av_default_container_wrap container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-13870'><div class='entry-content-wrapper clearfix'><div  class='flex_column av-av_one_full-7714eba142aeda1f8b5272a3c372b279 av_one_full  avia-builder-el-1  el_after_av_section  el_before_av_section  avia-builder-el-no-sibling  first flex_column_div  '     ><section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><p>The goal of conservation breeding is to breed animals in controlled environments for the purpose of releasing them back into the wild. When it comes to breeding animals in captivity, there are usually difficulties and issues. These challenges can be the result of many factors, such as lack of knowledge of their breeding behaviour, enclosure requirements, and special dietary needs.<\/p>\n<p>Solving the conservation breeding issues\u00a0 are critical, especially when dealing with species that are not commonly bred in captivity, such as endangered amphibians. Amphibians in captivity can face many reproductive dysfunctions. These dysfunctions in frogs and toads depend on multiple factors, including gamete (sperm and egg) quality in both sexes, genetics, husbandry and missing environmental stimuli that trigger reproduction. Either alone or in combination, these are the root causes often cited for the total or partial inhibition of reproduction currently observed in the majority of captive amphibian populations. For this reason, much research is required to determine and fix these dysfunctions.<\/p>\n<\/div><\/section>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-la2z9z97-7ba27fffdeaef5cb397cde0a16b43eb1\">\n.avia-image-container.av-la2z9z97-7ba27fffdeaef5cb397cde0a16b43eb1 img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-la2z9z97-7ba27fffdeaef5cb397cde0a16b43eb1 .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-la2z9z97-7ba27fffdeaef5cb397cde0a16b43eb1 av-styling- avia-align-center  avia-builder-el-3  el_after_av_textblock  el_before_av_textblock '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img fetchpriority=\"high\" class='wp-image-24366 avia-img-lazy-loading-not-24366 avia_image ' src='https:\/\/wildlifepreservation.ca\/wp-content\/uploads\/2016\/05\/oregonspottedfrog-300x195.jpg' alt='' title='oregonspottedfrog'  height=\"195\" width=\"300\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><p>WPC\u2019s <a href=\"https:\/\/wildlifepreservation.ca\/fraser-valley-wetlands-wildlife\/\">breeding program for Canada&#8217;s most endangered amphibian, the Oregon spotted frog<\/a> (<em>Rana pretiosa<\/em>), OSF for short, certainly presents its challenges. Fortunately, with the help of our partners at Toronto Zoo, we are working to better understand and solve these challenges. Part of this project is analyzing OSF&#8217;s sperm quality and factors that are affecting its quality.<\/p>\n<p><strong><em>The key question that might come to mind is &#8211; how do we collect sperm from a frog?<\/em><\/strong><\/p>\n<p>Well, it is an interesting process. Firstly, most frogs are external fertilizers; hence they do not have an external organ dedicated to breeding. Secondly, frogs usually release sperm while they urinate, so in order to collect sperm, we need to gather a sample of the frog\u2019s urine. Usually, biologists collect urine with a pipet from outside the cloaca or in our case, we insert catheters and collect urine directly from the cloaca. These methods may differ from one species to another.<\/p>\n<p>However in OSF sperm is not present in the urine when collected by this means. To make sure we have enough sperm for analysis, we use hormones that trigger the frogs to release sperm. The two hormones biologists mostly use in breeding programs are Human Chorionic Gonadotropin (HCG) and luteinizing hormone-releasing hormone (LHRH), also known as Gonadotropin-releasing hormone (GnRH). Biologists usually use either one or a combination of the two hormones. The dosage of hormones may differ between species, and for most species such as the OSF, the effect of different dosages of hormones is currently unknown.<\/p>\n<\/div><\/section>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-la2z9z97-cc8dd39921d0e775169b067d2881e2fc\">\n.avia-image-container.av-la2z9z97-cc8dd39921d0e775169b067d2881e2fc img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-la2z9z97-cc8dd39921d0e775169b067d2881e2fc .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-la2z9z97-cc8dd39921d0e775169b067d2881e2fc av-styling- avia-align-center  avia-builder-el-5  el_after_av_textblock  el_before_av_textblock '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img fetchpriority=\"high\" class='wp-image-33055 avia-img-lazy-loading-not-33055 avia_image ' src='https:\/\/wildlifepreservation.ca\/wp-content\/uploads\/2021\/04\/Pourya_sardari-sampling-263x300.jpg' alt='' title='Pourya_sardari sampling'  height=\"300\" width=\"263\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><p><em>Pourya Sardari sampling sperm from an Oregon spotted frog<\/em><\/p>\n<p>This breeding season we are completing research into this process. The purpose of the current project, which is still in its early stages, is to determine what will happen to OSF&#8217;s sperm at different hormone dosages.<\/p>\n<p>Conservation breeding can be quite challenging, especially with species that are not very common. Fortunately, with the help of science and technologies, we can improve the captive breeding programs&#8217; outcomes, producing more and healthier individuals to release back into the wild.<\/p>\n<\/div><\/section><\/p><\/div><\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='av_section_2'  class='avia-section av-1j97qu8-d954dbdec10ff5de904045a47b708e65 main_color avia-section-default avia-no-border-styling  avia-builder-el-7  el_after_av_one_full  el_before_av_section  avia-bg-style-scroll container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-13870'><div class='entry-content-wrapper clearfix'><\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='av_section_3'  class='avia-section av-1j97qu8-d954dbdec10ff5de904045a47b708e65 main_color avia-section-default avia-no-border-styling  avia-builder-el-8  el_after_av_section  el_before_av_one_third  avia-bg-style-scroll container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-13870'><div class='entry-content-wrapper clearfix'><\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='after_section_3'  class='main_color av_default_container_wrap container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-13870'><div class='entry-content-wrapper clearfix'><div  class='flex_column av-av_one_third-7714eba142aeda1f8b5272a3c372b279 av_one_third  avia-builder-el-9  el_after_av_section  el_before_av_two_third  avia-builder-el-first  first flex_column_div  '     ><style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-la2z9z97-22250bd55c6bf75073363f1a7f1c05cb\">\n.avia-image-container.av-la2z9z97-22250bd55c6bf75073363f1a7f1c05cb img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-la2z9z97-22250bd55c6bf75073363f1a7f1c05cb .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-la2z9z97-22250bd55c6bf75073363f1a7f1c05cb av-styling- avia-align-center  avia-builder-el-10  avia-builder-el-no-sibling '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img fetchpriority=\"high\" class='wp-image-30520 avia-img-lazy-loading-not-30520 avia_image ' src='https:\/\/wildlifepreservation.ca\/wp-content\/uploads\/2020\/06\/Andrea-gielens-for-bio-300x300.jpg' alt='' title='Andrea-gielens-for-bio'  height=\"300\" width=\"300\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><\/div><div  class='flex_column av-av_two_third-f1c518e9ff58e869f80c8f093487abc9 av_two_third  avia-builder-el-11  el_after_av_one_third  el_before_av_section  avia-builder-el-last  flex_column_div  '     ><section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><h3 class=\"et_pb_module_header\">Andrea Gielens<\/h3>\n<p class=\"et_pb_member_position\"><em><strong>Lead Biologist &#8211; Fraser Valley Wetlands Recovery Program<br \/> <\/strong><\/em><\/p>\n<p><span>Andrea manages WPC&#8217;s captive breeding and release programs for the Oregon spotted frog and the coastal western painted turtle. Andrea has studied at-risk reptiles and amphibians in Canada and abroad, including a term at the Durrell Wildlife Conservation Trust in Jersey. Andrea also manages the Taylor&#8217;s checkerspot butterfly recovery program in BC.<\/span><span><\/span><\/p>\n<\/div><\/section><\/div><\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='av_section_4'  class='avia-section av-1j97qu8-d954dbdec10ff5de904045a47b708e65 main_color avia-section-default avia-no-border-styling  avia-builder-el-13  el_after_av_two_third  el_before_av_section  avia-bg-style-scroll container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-13870'><div class='entry-content-wrapper clearfix'><\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='av_section_5'  class='avia-section av-1j97qu8-d954dbdec10ff5de904045a47b708e65 main_color avia-section-default avia-no-border-styling  avia-builder-el-14  el_after_av_section  el_before_av_one_full  avia-bg-style-scroll container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-13870'><div class='entry-content-wrapper clearfix'><\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='after_section_5'  class='main_color av_default_container_wrap container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-13870'><div class='entry-content-wrapper clearfix'><div  class='flex_column av-av_one_full-7714eba142aeda1f8b5272a3c372b279 av_one_full  avia-builder-el-15  el_after_av_section  el_before_av_textblock  avia-builder-el-first  first flex_column_div  '     ><section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><h2 style=\"text-align: center\"><span style=\"color: #000000\"><strong><\/strong><\/span><\/h2>\n<h2 style=\"text-align: center\"><span style=\"color: #000000\"><strong><\/strong><\/span><\/h2>\n<h2 style=\"text-align: center\"><span style=\"color: #ffffff\"><strong>Make a Donation<\/strong><\/span><\/h2>\n<p style=\"text-align: center\"><span style=\"color: #ffffff\">Show your support for endangered animals today. Your gift will go directly to our work on the front line of animal conservation.<\/span><\/p>\n<\/div><\/section><section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><p>    <section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><\/div><\/section><br \/>\n    [et_pb_code _builder_version=&#8221;4.2.2&#8243;]<\/p>\n<\/div><\/section><br \/>\n[\/av_textblock]<\/p>\n<div id=\"467538ea-f9a2-4223-ac13-ea83e3e5a0e0\"><!-- &#091;av_textblock textblock_styling_align='' textblock_styling='' textblock_styling_gap='' textblock_styling_mobile='' size='' av-desktop-font-size='' av-medium-font-size='' av-small-font-size='' av-mini-font-size='' font_color='' color='' id='' custom_class='' template_class='' av_uid='av-la2zajwc' sc_version='1.0' admin_preview_bg=''&#093;\n    &#091;av_textblock textblock_styling_align='' textblock_styling='' textblock_styling_gap='' textblock_styling_mobile='' size='' av-desktop-font-size='' av-medium-font-size='' av-small-font-size='' av-mini-font-size='' font_color='' color='' id='' custom_class='' template_class='' av_uid='av-la2zajwc' sc_version='1.0' admin_preview_bg=''&#093;\n    &#091;av_textblock textblock_styling_align='' textblock_styling='' textblock_styling_gap='' textblock_styling_mobile='' size='' av-desktop-font-size='' av-medium-font-size='' av-small-font-size='' av-mini-font-size='' font_color='' color='' id='' custom_class='' template_class='' av_uid='av-la2zajwc' sc_version='1.0' admin_preview_bg=''&#093;\n    &#091;av_textblock textblock_styling_align='' textblock_styling='' textblock_styling_gap='' textblock_styling_mobile='' size='' av-desktop-font-size='' av-medium-font-size='' av-small-font-size='' av-mini-font-size='' font_color='' color='' id='' custom_class='' template_class='' av_uid='av-la2zajwc' sc_version='1.0' admin_preview_bg=''&#093;\n    &#091;av_textblock textblock_styling_align='' textblock_styling='' textblock_styling_gap='' textblock_styling_mobile='' size='' av-desktop-font-size='' av-medium-font-size='' av-small-font-size='' av-mini-font-size='' font_color='' color='' id='' custom_class='' template_class='' av_uid='av-la2zajwc' sc_version='1.0' admin_preview_bg=''&#093;\n    &#091;av_textblock textblock_styling_align='' textblock_styling='' textblock_styling_gap='' textblock_styling_mobile='' size='' av-desktop-font-size='' av-medium-font-size='' av-small-font-size='' av-mini-font-size='' font_color='' color='' id='' custom_class='' template_class='' av_uid='av-la2zajwc' sc_version='1.0' admin_preview_bg=''&#093;\n    &#091;et_pb_line_break_holder&#093;\n&#091;\/av_textblock&#093;\n&#091;\/av_textblock&#093;\n&#091;\/av_textblock&#093;\n&#091;\/av_textblock&#093;\n&#091;\/av_textblock&#093;\n&#091;\/av_textblock&#093; 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