{"id":14190,"date":"2021-05-06T09:17:32","date_gmt":"2021-05-06T14:17:32","guid":{"rendered":"https:\/\/wildlifepreservation.qa.caorda.com\/blog\/?p=14190"},"modified":"2023-05-02T12:39:47","modified_gmt":"2023-05-02T17:39:47","slug":"counting-tadpoles-one-two-skip-a-few-ninety-nine-20-thousand","status":"publish","type":"post","link":"https:\/\/wildlifepreservation.ca\/fr\/blog\/counting-tadpoles-one-two-skip-a-few-ninety-nine-20-thousand\/","title":{"rendered":"Counting tadpoles &#8211; one, two, skip a few, ninety-nine, 20 thousand!"},"content":{"rendered":"\n<div id='av_section_1'  class='avia-section av-1j97qu8-d954dbdec10ff5de904045a47b708e65 main_color avia-section-default avia-no-border-styling  avia-builder-el-0  el_before_av_one_full  avia-builder-el-first  avia-bg-style-scroll container_wrap fullsize'  ><div class='container av-section-cont-open' ><main  role=\"main\" itemprop=\"mainContentOfPage\"  class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-14190'><div class='entry-content-wrapper clearfix'><\/div><\/div><\/main><!-- close content main element --><\/div><\/div><div id='after_section_1'  class='main_color av_default_container_wrap container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-14190'><div class='entry-content-wrapper clearfix'><div  class='flex_column av-av_one_full-7714eba142aeda1f8b5272a3c372b279 av_one_full  avia-builder-el-1  el_after_av_section  el_before_av_section  avia-builder-el-no-sibling  first flex_column_div  '     ><section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><p><em style=\"margin: 0px;padding: 0px;border: 0px;background: 0px 0px #ffffff;font-size: 14px;vertical-align: baseline;font-style: italic;color: #50863d;font-family: 'Open Sans', Arial, sans-serif;font-weight: 500;letter-spacing: normal;text-align: left;text-indent: 0px;text-transform: none\">\u00a0The\u00a0<a href=\"https:\/\/wildlifepreservation.ca\/2016\/oregon-spotted-frog\/\" style=\"margin: 0px;padding: 0px;border: 0px;background: 0px 0px;font-size: 14px;vertical-align: baseline;color: #83a56a;text-decoration: none\">Oregon spotted frog\u00a0<\/a>is Canada\u2019s most\u00a0<a href=\"https:\/\/wildlifepreservation.ca\/glossary\/endangered\/\" style=\"margin: 0px;padding: 0px;border: 0px;background: 0px 0px;font-size: 14px;vertical-align: baseline;color: #83a56a;text-decoration: none\">endangered<\/a>\u00a0frog, with fewer than 300 breeding individuals in the wild.\u00a0The recovery plan for Oregon spotted frog calls for\u00a0<a href=\"https:\/\/wildlifepreservation.ca\/glossary\/conservation-breeding\/\" style=\"margin: 0px;padding: 0px;border: 0px;background: 0px 0px;font-size: 14px;vertical-align: baseline;color: #83a56a;text-decoration: none\">conservation breeding<\/a>,\u00a0<a href=\"https:\/\/wildlifepreservation.ca\/glossary\/headstarting\/\" style=\"margin: 0px;padding: 0px;border: 0px;background: 0px 0px;font-size: 14px;vertical-align: baseline;color: #83a56a;text-decoration: none\">headstarting<\/a>\u00a0and release to re-establish viable populations in at least six sites. Wildlife Preservation Canada began coordinating these efforts in 2010, adding a\u00a0<a href=\"https:\/\/wildlifepreservation.ca\/glossary\/conservation-breeding\/\" style=\"margin: 0px;padding: 0px;border: 0px;background: 0px 0px;font-size: 14px;vertical-align: baseline;color: #83a56a;text-decoration: none\">conservation breeding<\/a>\u00a0program to the\u00a0<a href=\"https:\/\/wildlifepreservation.ca\/glossary\/headstarting\/\" style=\"margin: 0px;padding: 0px;border: 0px;background: 0px 0px;font-size: 14px;vertical-align: baseline;color: #83a56a;text-decoration: none\">headstarting<\/a>\u00a0work taking place at the Greater Vancouver Zoo. This expanded on the breeding efforts underway at the Vancouver Aquarium. <br \/><\/em><\/p>\n<\/div><\/section><section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><p>    [et_pb_video src=&#8221;https:\/\/wildlifepreservation.ca\/blog\/wp-content\/uploads\/2021\/05\/tads-in-a-bucket.mov&#8221; _builder_version=&#8221;4.2.2&#8243; hover_enabled=&#8221;0&#8243;]<\/p>\n<\/div><\/section><section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><p>    [\/et_pb_video]<\/p>\n<\/div><\/section><section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><p><em>Three staff from the Oregon spotted frog recovery team spent five days counting tadpoles, coming up with a whopping 20,514 tadpoles! (Give or take a few &#8211; you can see how this could be a difficult task!)<br \/> <\/em><\/p>\n<p>We had amazing success with our egg laying females this year with 41 out of 43 successfully laying their eggs! (<a href=\"https:\/\/wildlifepreservation.ca\/blog\/booming-year-for-frog-eggs\/\" target=\"_blank\" rel=\"noopener noreferrer\">Read more about how we helped this happen, what we changed and our ideas why <u>here<\/u><\/a>). This is great for our program. It means we have learned at the very least the \u201chow\u201d portion of getting females to release their eggs, even if the \u201cwhy\u201d is yet to be determined. Getting females to lay their eggs means that we will be able to minimize the number of females with egg binding, where the females retains their eggs through the end of the breeding season. Egg binding either results in infection and ultimately death or would require risky surgery which renders the females sterile. Getting eggs out of the females reliably was step number one.<\/p>\n<p>Now, we dream bigger! What if those eggs are actually fertile? In the past we have had poor fertility in the captive program, with most clusters under 10% fertility. This means that in a typical year, even if we get 30+ egg masses we would see only around 2000 tadpoles. When we are looking at breeding endangered species low fertility could be caused by on overwhelming number of factors.<\/p>\n<ul>\n<li>Is the quality of eggs and sperm poor?<\/li>\n<li>Is the water chemistry off? If so how is it off?<\/li>\n<li>What are the captive compared to wild water parameters?<\/li>\n<li>Is the nutrition of the frogs not appropriate? If so how?<\/li>\n<li>At what life stage is nutrition most important?<\/li>\n<li>Are the genetics of the captive population inbred or otherwise not compatible?<\/li>\n<\/ul>\n<p>And the list goes on!<\/p>\n<p>Once the eggs are laid all we can do is wait and see. Luckily for us, tadpole eggs are massive compared to some other species, and a lot easier to see since they are external to the animal and encased in clear jelly. In fact we can closely track the stages of the developing embryo through a process called Gosner Staging. This key below helps us to see the sequential progression of the eggs through the defined stages and compare between clutches. So once we have eggs we wait with baited breath, for the signs of development.<\/p>\n<\/div><\/section>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-la2z9z97-9da83cbc2702b66d0aeb497a75619a43\">\n.avia-image-container.av-la2z9z97-9da83cbc2702b66d0aeb497a75619a43 img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-la2z9z97-9da83cbc2702b66d0aeb497a75619a43 .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-la2z9z97-9da83cbc2702b66d0aeb497a75619a43 av-styling- avia-align-center  avia-builder-el-6  el_after_av_textblock  el_before_av_textblock '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img fetchpriority=\"high\" class='wp-image-33251 avia-img-lazy-loading-not-33251 avia_image ' src='https:\/\/wildlifepreservation.ca\/wp-content\/uploads\/2021\/05\/Gosner-stage-255x300.png' alt='' title='Gosner stage'  height=\"300\" width=\"255\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><p><em>The Gosner Staging System for Anurans, an amphibian development chart<br \/> <\/em><\/p>\n<p>Soon we start to see changes. We were thrown off by the very first egg mass where there was hardly any development. Disappointed, we thought, &#8220;Oh well, at least we learned how to get the females to lay eggs reliably, that\u2019s a huge achievement.&#8221;<\/p>\n<p>But\u2026.wait. The first egg mass was a dud, but the next ones are getting a blastopore, which looks like a white blotch (about stage 10), and cell cleavage. Soon the rest of the egg masses had developed to what we affectionately call the \u201cjelly bean\u201d stage (stage 15). And then we thought to ourselves\u2026is this actually happening?!?<\/p>\n<p>Yes, yes, it is! Well, we thought, &#8220;At least its April, the weather will be cool and development will be slow. As they start to hatch we can slowly count them and release them, since we can\u2019t possibly rear them all.&#8221;<\/p>\n<p>But the weather forecast said &#8211; haha yeah right, how about summer in April instead! Daytime temperatures shot to 25+ and nights above 15. This meant our bounty of tadpoles grew very fast, hatched in record speed, and all at once.<\/p>\n<p>The team sprang into action. We arranged a release day in 5 days from first hatch and began to count tadpoles. Three staff members spent the next 5 days counting. Within the first day we had already counted more tadpoles than we would have in a normal year, over 3000, and we hadn\u2019t even made a dent. The next days we would see at least 4000 more per day. If you\u2019re doing the math with me you can see that number going up.<\/p>\n<p>Once the dust had settled and the spread sheet was compiled we had counted a <strong>whopping 20,514 tadpoles<\/strong>, give or take a few. It is generally accepted that to establish a species like a frog, it is necessary to release 5,000 animals a year for 5 years. <strong>Our production of 20,000 tadpoles this year alone is double the number we released in total over the past 10 years!<\/strong> We are in a much better position to establish new populations of this species as well as supplement exiting populations of this frog.<\/p>\n<p><strong>The release of these animals into our restored site dwarfs all previous releases at this site\u2026combined. <br \/><\/strong><\/p>\n<p>Now with tadpoles counted and 1000 retained to rear throughout summer, we take a deep breath and prepare to drive our precious cargo to the release site for their big day. Stay tuned for our release day blog!<\/p>\n<p>Does anyone know where I can get 20,000 tiny seat belts?<\/p>\n<\/div><\/section><\/p><\/div><\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='av_section_2'  class='avia-section av-1j97qu8-d954dbdec10ff5de904045a47b708e65 main_color avia-section-default avia-no-border-styling  avia-builder-el-8  el_after_av_one_full  el_before_av_section  avia-bg-style-scroll container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-14190'><div class='entry-content-wrapper clearfix'><\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='av_section_3'  class='avia-section av-1j97qu8-d954dbdec10ff5de904045a47b708e65 main_color avia-section-default avia-no-border-styling  avia-builder-el-9  el_after_av_section  el_before_av_one_third  avia-bg-style-scroll container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-14190'><div class='entry-content-wrapper clearfix'><\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='after_section_3'  class='main_color av_default_container_wrap container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-14190'><div class='entry-content-wrapper clearfix'><div  class='flex_column av-av_one_third-7714eba142aeda1f8b5272a3c372b279 av_one_third  avia-builder-el-10  el_after_av_section  el_before_av_two_third  avia-builder-el-first  first flex_column_div  '     ><style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-la2z9z97-22250bd55c6bf75073363f1a7f1c05cb\">\n.avia-image-container.av-la2z9z97-22250bd55c6bf75073363f1a7f1c05cb img.avia_image{\nbox-shadow:none;\n}\n.avia-image-container.av-la2z9z97-22250bd55c6bf75073363f1a7f1c05cb .av-image-caption-overlay-center{\ncolor:#ffffff;\n}\n<\/style>\n<div  class='avia-image-container av-la2z9z97-22250bd55c6bf75073363f1a7f1c05cb av-styling- avia-align-center  avia-builder-el-11  avia-builder-el-no-sibling '   itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\" ><div class=\"avia-image-container-inner\"><div class=\"avia-image-overlay-wrap\"><img fetchpriority=\"high\" class='wp-image-30520 avia-img-lazy-loading-not-30520 avia_image ' src='https:\/\/wildlifepreservation.ca\/wp-content\/uploads\/2020\/06\/Andrea-gielens-for-bio-300x300.jpg' alt='' title='Andrea-gielens-for-bio'  height=\"300\" width=\"300\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><\/div><div  class='flex_column av-av_two_third-f1c518e9ff58e869f80c8f093487abc9 av_two_third  avia-builder-el-12  el_after_av_one_third  el_before_av_section  avia-builder-el-last  flex_column_div  '     ><section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><h3 class=\"et_pb_module_header\">Andrea Gielens<\/h3>\n<p class=\"et_pb_member_position\"><em><strong>Lead Biologist &#8211; Fraser Valley Wetlands Recovery Program<br \/><\/strong><\/em><\/p>\n<p><span>Andrea manages WPC&#8217;s captive breeding and release programs for the Oregon spotted frog and the coastal western painted turtle. Andrea has studied at-risk reptiles and amphibians in Canada and abroad, including a term at the Durrell Wildlife Conservation Trust in Jersey. Andrea also manages the Taylor&#8217;s checkerspot butterfly recovery program in BC.<\/span><span><\/span><\/p>\n<\/div><\/section><\/div><\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='av_section_4'  class='avia-section av-1j97qu8-d954dbdec10ff5de904045a47b708e65 main_color avia-section-default avia-no-border-styling  avia-builder-el-14  el_after_av_two_third  el_before_av_section  avia-bg-style-scroll container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-14190'><div class='entry-content-wrapper clearfix'><\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='av_section_5'  class='avia-section av-1j97qu8-d954dbdec10ff5de904045a47b708e65 main_color avia-section-default avia-no-border-styling  avia-builder-el-15  el_after_av_section  el_before_av_one_full  avia-bg-style-scroll container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-14190'><div class='entry-content-wrapper clearfix'><\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='after_section_5'  class='main_color av_default_container_wrap container_wrap fullsize'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-14190'><div class='entry-content-wrapper clearfix'><div  class='flex_column av-av_one_full-7714eba142aeda1f8b5272a3c372b279 av_one_full  avia-builder-el-16  el_after_av_section  el_before_av_textblock  avia-builder-el-first  first flex_column_div  '     ><section  class='av_textblock_section av-la2zajwc-ee03232185bfde5f783df419b9aded77 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/BlogPosting\" itemprop=\"blogPost\" ><div class='avia_textblock'  itemprop=\"text\" ><h2 style=\"text-align: center\"><\/h2>\n<h2 style=\"text-align: center\"><\/h2>\n<h2 style=\"text-align: center\"><span style=\"color: #ffffff\"><strong>Make a Donation<\/strong><\/span><\/h2>\n<p style=\"text-align: center\"><span style=\"color: #ffffff\">Show your support for endangered animals today. 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